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Generating human intestinal tissue from pluripotent stem cells in vitro
We describe a protocol to generate 3-dimensional human intestinal tissue (called organoids) in vitro from human pluripotent stem cells. To generate intestinal organoids, pluripotent stem cells are first differentiated into FOXA2+/SOX17+ endoderm by treating the cells with ActivinA for 3 days. Follow...
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| Main Authors: | , , , |
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| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
2011
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3896236/ https://ncbi.nlm.nih.gov/pubmed/22082986 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2011.410 |
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