Generating human intestinal tissue from pluripotent stem cells in vitro

We describe a protocol to generate 3-dimensional human intestinal tissue (called organoids) in vitro from human pluripotent stem cells. To generate intestinal organoids, pluripotent stem cells are first differentiated into FOXA2+/SOX17+ endoderm by treating the cells with ActivinA for 3 days. Follow...

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Bibliographic Details
Main Authors: McCracken, Kyle W., Howell, Jonathan C., Wells, James M., Spence, Jason R.
Format: Artigo
Language:Inglês
Published: 2011
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Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3896236/
https://ncbi.nlm.nih.gov/pubmed/22082986
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2011.410
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