High-throughput platform for real-time monitoring of biological processes by multicolor single-molecule fluorescence

Zero-mode waveguides provide a powerful technology for studying single-molecule real-time dynamics of biological systems at physiological ligand concentrations. We customized a commercial zero-mode waveguide-based DNA sequencer for use as a versatile instrument for single-molecule fluorescence detec...

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Bibliographische Detailangaben
Hauptverfasser: Chen, Jin, Dalal, Ravindra V., Petrov, Alexey N., Tsai, Albert, O’Leary, Seán E., Chapin, Karen, Cheng, Janice, Ewan, Mark, Hsiung, Pei-Lin, Lundquist, Paul, Turner, Stephen W., Hsu, David R., Puglisi, Joseph D.
Format: Artigo
Sprache:Inglês
Veröffentlicht: National Academy of Sciences 2014
Schlagworte:
Biological Sciences
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3896158/
https://ncbi.nlm.nih.gov/pubmed/24379388
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1315735111
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