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Release, Identification, and Isolation of Messenger RNA from Mammalian Ribosomes

The puromycin-induced dissociation, at high ionic strength, of active ribosomes from reticulocytes resulted in the release of protein-free, apparently undegraded, messenger RNA for globin, which was identified by centrifugation on a sucrose density gradient. This procedure should make messenger RNA...

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Bibliographic Details
Main Author: Blobel, Günter
Format: Artigo
Language:Inglês
Published: 1971
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC389054/
https://ncbi.nlm.nih.gov/pubmed/5279524
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