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Release, Identification, and Isolation of Messenger RNA from Mammalian Ribosomes

The puromycin-induced dissociation, at high ionic strength, of active ribosomes from reticulocytes resulted in the release of protein-free, apparently undegraded, messenger RNA for globin, which was identified by centrifugation on a sucrose density gradient. This procedure should make messenger RNA...

Ausführliche Beschreibung

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Bibliografische Detailangaben
Veröffentlicht in:Proc Natl Acad Sci U S A
1. Verfasser: Blobel, Günter
Format: Artigo
Sprache:Inglês
Veröffentlicht: National Academy of Sciences 1971
Schlagworte:
Online-Zugang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC389054/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/5279524/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.68.4.832
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