Dissociation of Mammalian Polyribosomes into Subunits by Puromycin
Hepatic ribosomes have been dissociated into biologically active subunits as follows. Polysomes were treated at 0°C with puromycin at high ionic strength. This released most of the nascent polypeptide chains without dissociating the polysomes, which retained the mRNA and the tRNA moiety of peptidyl...
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| Publicado en: | Proc Natl Acad Sci U S A |
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| Autores principales: | , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
National Academy of Sciences
1971
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC388945/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/5277091/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.68.2.390 |
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