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Dissociation of Mammalian Polyribosomes into Subunits by Puromycin

Hepatic ribosomes have been dissociated into biologically active subunits as follows. Polysomes were treated at 0°C with puromycin at high ionic strength. This released most of the nascent polypeptide chains without dissociating the polysomes, which retained the mRNA and the tRNA moiety of peptidyl...

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Dettagli Bibliografici
Autori principali: Blobel, Günter, Sabatini, David
Natura: Artigo
Lingua:Inglês
Pubblicazione: 1971
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC388945/
https://ncbi.nlm.nih.gov/pubmed/5277091
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