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A new thermodynamic model describes the effects of ligand density and type, salt concentration and protein species in hydrophobic interaction chromatography

A new thermodynamic model is derived that describes both loading and pulse-response behavior of proteins in hydrophobic interaction chromatography (HIC). The model describes adsorption in terms of protein and solvent activities, and water displacement from hydrophobic interfaces, and distinguishes c...

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Detalhes bibliográficos
Main Authors: Deitcher, R.W., Rome, J.E., Gildea, P.A., O’Connell, J.P., Fernandez, E.J.
Formato: Artigo
Idioma:Inglês
Publicado em: 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3890380/
https://ncbi.nlm.nih.gov/pubmed/19695574
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.chroma.2009.07.068
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