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A new thermodynamic model describes the effects of ligand density and type, salt concentration and protein species in hydrophobic interaction chromatography

A new thermodynamic model is derived that describes both loading and pulse-response behavior of proteins in hydrophobic interaction chromatography (HIC). The model describes adsorption in terms of protein and solvent activities, and water displacement from hydrophobic interfaces, and distinguishes c...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Deitcher, R.W., Rome, J.E., Gildea, P.A., O’Connell, J.P., Fernandez, E.J.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2009
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3890380/
https://ncbi.nlm.nih.gov/pubmed/19695574
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.chroma.2009.07.068
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