A Simple, Sensitive Protein-binding Assay for Guanosine 3′:5′-Monophosphate
A protein-binding assay for guanosine 3′:5′-monophosphate, which employs an enzyme preparation from lobster tail muscle, is described. The binding constant for cyclic GMP was 2-10 nM for four different enzyme preparations. The method can be set up in several days and permits the detection of as litt...
Uloženo v:
| Vydáno v: | Proc Natl Acad Sci U S A |
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| Hlavní autoři: | , , |
| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
National Academy of Sciences
1971
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC389031/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4323786/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.68.4.736 |
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