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A Simple, Sensitive Protein-binding Assay for Guanosine 3′:5′-Monophosphate

A protein-binding assay for guanosine 3′:5′-monophosphate, which employs an enzyme preparation from lobster tail muscle, is described. The binding constant for cyclic GMP was 2-10 nM for four different enzyme preparations. The method can be set up in several days and permits the detection of as litt...

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Detalles Bibliográficos
Main Authors: Murad, Ferid, Manganiello, Vincent, Vaughan, Martha
Formato: Artigo
Idioma:Inglês
Publicado: 1971
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC389031/
https://ncbi.nlm.nih.gov/pubmed/4323786
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