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A Simple, Sensitive Protein-binding Assay for Guanosine 3′:5′-Monophosphate

A protein-binding assay for guanosine 3′:5′-monophosphate, which employs an enzyme preparation from lobster tail muscle, is described. The binding constant for cyclic GMP was 2-10 nM for four different enzyme preparations. The method can be set up in several days and permits the detection of as litt...

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Veröffentlicht in:Proc Natl Acad Sci U S A
Hauptverfasser: Murad, Ferid, Manganiello, Vincent, Vaughan, Martha
Format: Artigo
Sprache:Inglês
Veröffentlicht: National Academy of Sciences 1971
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC389031/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4323786/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.68.4.736
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