Rapid conversion of EUCOMM/KOMP-CSD alleles in mouse embryos using a cell-permeable Cre recombinase

We describe here use of a cell-permeable Cre to efficiently convert the EUCOMM/KOMP-CSD tm1a allele to the tm1b form in preimplantation mouse embryos in a high-throughput manner, consistent with the requirements of the International Mouse Phenotyping Consortium-affiliated NIH KOMP2 project. This met...

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Bibliografiske detaljer
Main Authors: Ryder, Edward, Doe, Brendan, Gleeson, Diane, Houghton, Richard, Dalvi, Priya, Grau, Evelyn, Habib, Bishoy, Miklejewska, Evelina, Newman, Stuart, Sethi, Debarati, Sinclair, Caroline, Vyas, Sapna, Wardle-Jones, Hannah, Bottomley, Joanna, Bussell, James, Galli, Antonella, Salisbury, Jennifer, Ramirez-Solis, Ramiro
Format: Artigo
Sprog:Inglês
Udgivet: Springer Netherlands 2013
Fag:
Technical Report
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3890051/
https://ncbi.nlm.nih.gov/pubmed/24197666
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s11248-013-9764-x
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