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Genomic targeting with purified Cre recombinase.

Purified Cre recombinase protein introduced directly into cultured mammalian cells by lipofection catalyzes both site-specific chromosomal integration of a co-transfected lox targeting vector and precise excision of genomic DNA flanked by directly repeated lox sites. This procedure eliminates the ne...

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Detalhes bibliográficos
Main Authors: Baubonis, W, Sauer, B
Formato: Artigo
Idioma:Inglês
Publicado em: 1993
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC309460/
https://ncbi.nlm.nih.gov/pubmed/8502542
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