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Genomic targeting with purified Cre recombinase.
Purified Cre recombinase protein introduced directly into cultured mammalian cells by lipofection catalyzes both site-specific chromosomal integration of a co-transfected lox targeting vector and precise excision of genomic DNA flanked by directly repeated lox sites. This procedure eliminates the ne...
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| 主要な著者: | , |
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| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
1993
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| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC309460/ https://ncbi.nlm.nih.gov/pubmed/8502542 |
| タグ: |
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