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Live-cell fluorescence imaging reveals high stoichiometry of Grb2 binding to the EGF receptor sustained during endocytosis

Activation of epidermal growth factor (EGF) receptor (EGFR) leads to its interaction with Grb2, a dual-function adapter mediating both signaling through Ras and receptor endocytosis. We used time-lapse three-dimensional imaging by spinning disk confocal microscopy to analyze trafficking of EGFR and...

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Detalhes bibliográficos
Main Authors: Fortian, Arola, Sorkin, Alexander
Formato: Artigo
Idioma:Inglês
Publicado em: The Company of Biologists 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3889400/
https://ncbi.nlm.nih.gov/pubmed/24259669
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1242/jcs.137786
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