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Phage Display of the Serpin Alpha-1 Proteinase Inhibitor Randomized at Consecutive Residues in the Reactive Centre Loop and Biopanned with or without Thrombin

In spite of the power of phage display technology to identify variant proteins with novel properties in large libraries, it has only been previously applied to one member of the serpin superfamily. Here we describe phage display of human alpha-1 proteinase inhibitor (API) in a T7 bacteriophage syste...

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Detalhes bibliográficos
Main Authors: Scott, Benjamin M., Matochko, Wadim L., Gierczak, Richard F., Bhakta, Varsha, Derda, Ratmir, Sheffield, William P.
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3888415/
https://ncbi.nlm.nih.gov/pubmed/24427287
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0084491
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