A highly sensitive method for analysis of 7-dehydrocholesterol for the study of Smith-Lemli-Opitz syndrome

We describe a highly sensitive method for the detection of 7-dehydrocholesterol (7-DHC), the biosynthetic precursor of cholesterol, based on its reactivity with 4-phenyl-1,2,4-triazoline-3,5-dione (PTAD) in a Diels-Alder cycloaddition reaction. Samples of biological tissues and fluids with added deu...

Full description

Saved in:
Bibliographic Details
Main Authors: Liu, Wei, Xu, Libin, Lamberson, Connor, Haas, Dorothea, Korade, Zeljka, Porter, Ned A.
Format: Artigo
Language:Inglês
Published: The American Society for Biochemistry and Molecular Biology 2014
Subjects:
Methods
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3886672/
https://ncbi.nlm.nih.gov/pubmed/24259532
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1194/jlr.D043877
Tags: Add Tag
No Tags, Be the first to tag this record!

Similar Items