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Chromatographic assays of drug oxidation by human cytochrome P450 3A4
Cytochrome P450 enzymes (P450s) are heme-thiolate mono-oxygenases involved in the oxidation of many endogenous and exogenous substrates. Herein, we describe two protocols for measuring the activity of a key enzyme of drug metabolism, P450 3A4. In this protocol, the substrate is incubated with human...
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| 主要な著者: | , , |
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| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
2009
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3883453/ https://ncbi.nlm.nih.gov/pubmed/19661995 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2009.122 |
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