Integrative Network Analysis of Signaling in Human CD34+ Hematopoietic Progenitor Cells by Global Phosphoproteomic Profiling Using TiO2 Enrichment Combined with 2D LC-MS/MS and Pathway Mapping

Protein kinase signaling regulates human hematopoietic stem/progenitor cell (HSPC) fate, yet little is known about critical pathway substrates. To address this, we have developed and applied a large-scale, empirically-optimized phosphopeptide affinity enrichment strategy with high-throughput 2D LC-M...

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Detalhes bibliográficos
Main Authors: Guo, Hongbo, Isserlin, Ruth, Chen, Xiaoji, Wang, Weijia, Phanse, Sadhna, Zandstra, Peter W., Bader, Gary, Paddison, Patrick, Emili, Andrew
Formato: Artigo
Idioma:Inglês
Publicado em: 2013
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3883140/
https://ncbi.nlm.nih.gov/pubmed/23401153
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pmic.201200369
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