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An Improved Protocol for Intact Chloroplasts and cpDNA Isolation in Conifers
BACKGROUND: Performing chloroplast DNA (cpDNA) isolation is considered a major challenge among different plant groups, especially conifers. Isolating chloroplasts in conifers by such conventional methods as sucrose gradient and high salt has not been successful. So far, plastid genome sequencing pro...
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| Main Authors: | , , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Public Library of Science
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3879346/ https://ncbi.nlm.nih.gov/pubmed/24392157 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0084792 |
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