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NaOH low‐salt method for chloroplast isolation and highly pure cpDNA preparation from Aeluropus littoralis

PREMISE OF THE STUDY: High‐yield pure chloroplast DNA (cpDNA) is necessary for whole genome sequencing. Chloroplast extraction with traditional high‐salt methods causes damage to nuclei and destroys the integrity of organelles, which leads to high genomic contamination from the nucleus and mitochond...

Deskribapen osoa

Gorde:
Xehetasun bibliografikoak
Argitaratua izan da:Appl Plant Sci
Egile Nagusiak: Nasiri, Najmeh, Nematzadeh, Ghorbanali, Shariati‐Joni, Vahid, Najafi‐Zarrini, Hamid
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: John Wiley and Sons Inc. 2018
Gaiak:
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC6159644/
https://ncbi.nlm.nih.gov/pubmed/30276031
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/aps3.1183
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