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NaOH low‐salt method for chloroplast isolation and highly pure cpDNA preparation from Aeluropus littoralis
PREMISE OF THE STUDY: High‐yield pure chloroplast DNA (cpDNA) is necessary for whole genome sequencing. Chloroplast extraction with traditional high‐salt methods causes damage to nuclei and destroys the integrity of organelles, which leads to high genomic contamination from the nucleus and mitochond...
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| Pubblicato in: | Appl Plant Sci |
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| Autori principali: | , , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
John Wiley and Sons Inc.
2018
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6159644/ https://ncbi.nlm.nih.gov/pubmed/30276031 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/aps3.1183 |
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