An Improved Protocol for Intact Chloroplasts and cpDNA Isolation in Conifers

BACKGROUND: Performing chloroplast DNA (cpDNA) isolation is considered a major challenge among different plant groups, especially conifers. Isolating chloroplasts in conifers by such conventional methods as sucrose gradient and high salt has not been successful. So far, plastid genome sequencing pro...

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Main Authors: Vieira, Leila do Nascimento, Faoro, Helisson, Fraga, Hugo Pacheco de Freitas, Rogalski, Marcelo, de Souza, Emanuel Maltempi, de Oliveira Pedrosa, Fábio, Nodari, Rubens Onofre, Guerra, Miguel Pedro
格式: Artigo
語言:Inglês
出版: Public Library of Science 2014
主題:
Research Article
在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC3879346/
https://ncbi.nlm.nih.gov/pubmed/24392157
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0084792
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