Screening Fluorescent Voltage Indicators with Spontaneously Spiking HEK Cells

Development of improved fluorescent voltage indicators is a key challenge in neuroscience, but progress has been hampered by the low throughput of patch-clamp characterization. We introduce a line of non-fluorescent HEK cells that stably express Na(V) 1.3 and K(IR) 2.1 and generate spontaneous elect...

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Detalhes bibliográficos
Main Authors: Park, Jeehae, Werley, Christopher A., Venkatachalam, Veena, Kralj, Joel M., Dib-Hajj, Sulayman D., Waxman, Stephen G., Cohen, Adam E.
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2013
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3877367/
https://ncbi.nlm.nih.gov/pubmed/24391999
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0085221
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