Highly efficient gene knockout in mice and zebrafish with RNA-guided endonucleases

RNA-guided endonucleases (RGENs), derived from the prokaryotic Type II CRISPR-Cas system, enable targeted genome modification in cells and organisms. Here we describe the establishment of gene-knockout mice and zebrafish by the injection of RGENs as Cas9 protein:guide RNA complexes or Cas9 mRNA plus...

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Main Authors: Sung, Young Hoon, Kim, Jong Min, Kim, Hyun-Taek, Lee, Jaehoon, Jeon, Jisun, Jin, Young, Choi, Jung-Hwa, Ban, Young Ho, Ha, Sang-Jun, Kim, Cheol-Hee, Lee, Han-Woong, Kim, Jin-Soo
格式: Artigo
語言:Inglês
出版: Cold Spring Harbor Laboratory Press 2014
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Method
在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC3875853/
https://ncbi.nlm.nih.gov/pubmed/24253447
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/gr.163394.113
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