Functional reconstitution of the mitochondrial Ca(2+)/H(+) antiporter Letm1

The leucine zipper, EF hand–containing transmembrane protein 1 (Letm1) gene encodes a mitochondrial inner membrane protein, whose depletion severely perturbs mitochondrial Ca(2+) and K(+) homeostasis. Here we expressed, purified, and reconstituted human Letm1 protein in liposomes. Using Ca(2+) fluor...

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Bibliografiske detaljer
Main Authors: Tsai, Ming-Feng, Jiang, Dawei, Zhao, Linlin, Clapham, David, Miller, Christopher
Format: Artigo
Sprog:Inglês
Udgivet: The Rockefeller University Press 2014
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Research Articles
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3874562/
https://ncbi.nlm.nih.gov/pubmed/24344246
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1085/jgp.201311096
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