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Rewriting the rules for end joining via enzymatic splicing of DNA 3′-PO(4) and 5′-OH ends

There are many biological contexts in which DNA damage generates “dirty” breaks with 3′-PO(4) (or cyclic-PO(4)) and 5′-OH ends that cannot be sealed by DNA ligases. Here we show that the Escherichia coli RNA ligase RtcB can splice these dirty DNA ends via a unique chemical mechanism. RtcB transfers...

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Detalhes bibliográficos
Main Authors: Das, Ushati, Chakravarty, Anupam K., Remus, Barbara S., Shuman, Stewart
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3870732/
https://ncbi.nlm.nih.gov/pubmed/24218597
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1314289110
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