Resinless section electron microscopy of HeLa cell mitotic architecture.
The use of resinless sections extends embedment-free electron microscopy to the cytoskeleton of thick specimens. Here we examine HeLa cells rounded at mitosis. Extraction of mitotic HeLa cells with Triton X-100 removes lipids and soluble proteins, leaving the cytoskeletal framework and spindle appar...
Shranjeno v:
| izdano v: | Proc Natl Acad Sci U S A |
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| Principais autores: | , , |
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
National Academy of Sciences
1986
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC387061/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3466172/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.83.23.8996 |
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