Immunolocalization in three dimensions: immunogold staining of cytoskeletal and nuclear matrix proteins in resinless electron microscopy sections.

We describe two methods for staining resinless thin sections with antibodies and gold-conjugated second antibodies. Immunolocalization of specific proteins is a powerful tool for cell structure studies but current techniques do not develop its full potential. Immunofluorescence provides only low-res...

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Detalhes bibliográficos

Publicado no:	Proc Natl Acad Sci U S A
Principais autores:	Nickerson, J A, Krockmalnic, G, He, D C, Penman, S
Formato:	Artigo
Idioma:	Inglês
Publicado em:	National Academy of Sciences 1990
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC53666/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2315318/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.87.6.2259
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