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Imaging cytoskeleton--mitochondrial membrane attachments by embedment-free electron microscopy of saponin-extracted cells.

Embedment-free electron microscopy images the cytoskeleton and nuclear matrix, which are very difficult to visualize in conventional electron micrographs. However, to be effective, cell structures must be depleted of soluble proteins, which otherwise shroud cell architecture. Nonionic detergents eff...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Principais autores: Lin, A, Krockmalnic, G, Penman, S
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 1990
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC54997/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2236067/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.87.21.8565
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