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Resinless section electron microscopy of HeLa cell mitotic architecture.

The use of resinless sections extends embedment-free electron microscopy to the cytoskeleton of thick specimens. Here we examine HeLa cells rounded at mitosis. Extraction of mitotic HeLa cells with Triton X-100 removes lipids and soluble proteins, leaving the cytoskeletal framework and spindle appar...

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Bibliografski detalji
Glavni autori: Wagner, B, Krochmalnic, G, Penman, S
Format: Artigo
Jezik:Inglês
Izdano: 1986
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC387061/
https://ncbi.nlm.nih.gov/pubmed/3466172
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