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Enzyme-mediated spatial segregation on individual polymeric support beads: application to generation and screening of encoded combinatorial libraries.

Proteolysis of short N alpha-protected peptide substrates bound to polyoxyethylene-polystyrene beads releases selectively free amino sites in the enzyme-accessible "surface" area. The substantial majority of functional sites in the "interior" of the polymeric support are not reached by the enzyme an...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Principais autores: Vágner, J, Barany, G, Lam, K S, Krchnák, V, Sepetov, N F, Ostrem, J A, Strop, P, Lebl, M
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 1996
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC38645/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8710846/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.93.16.8194
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