Hydroxyl radical "footprinting": high-resolution information about DNA-protein contacts and application to lambda repressor and Cro protein.
A method has been developed for making "footprints" of proteins bound to DNA. The hydroxyl radical, generated by reduction of hydrogen peroxide by iron(II), is the reagent used to cut the DNA. Hydroxyl radical breaks the backbone of DNA with almost no sequence dependence, so all backbone positions m...
-д хадгалсан:
| -д хэвлэсэн: | Proc Natl Acad Sci U S A |
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| Үндсэн зохиолчид: | , |
| Формат: | Artigo |
| Хэл сонгох: | Inglês |
| Хэвлэсэн: |
National Academy of Sciences
1986
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| Нөхцлүүд: | |
| Онлайн хандалт: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC386308/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3090544/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.83.15.5469 |
| Шошгууд: |
Шошго байхгүй, Энэхүү баримтыг шошголох эхний хүн болох!
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