Evidence for a conformational change in the DNA gyrase-DNA complex from hydroxyl radical footprinting.
We have used the technique of hydroxyl radical footprinting to probe the complex between DNA gyrase and a 198 bp DNA fragment containing the preferred gyrase cleavage site from plasmid pBR322. We find that gyrase protects 128 bp from the hydroxyl radical with the central 13 bp (adjacent to the gyras...
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| Pubblicato in: | Nucleic Acids Res |
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| Autori principali: | , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Oxford University Press
1994
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| Accesso online: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC308031/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8202356/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/22.9.1567 |
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