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Evidence for a conformational change in the DNA gyrase-DNA complex from hydroxyl radical footprinting.

We have used the technique of hydroxyl radical footprinting to probe the complex between DNA gyrase and a 198 bp DNA fragment containing the preferred gyrase cleavage site from plasmid pBR322. We find that gyrase protects 128 bp from the hydroxyl radical with the central 13 bp (adjacent to the gyras...

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Main Authors: Orphanides, G, Maxwell, A
Formato: Artigo
Idioma:Inglês
Publicado: 1994
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC308031/
https://ncbi.nlm.nih.gov/pubmed/8202356
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