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Labeled Nucleoside Triphosphates with Reversibly Terminating Aminoalkoxyl Groups

Nucleoside triphosphates having a 3′-ONH(2) blocking group have been prepared with and without fluorescent tags on their nucleobases. DNA polymerases were identified that accepted these, adding a single nucleotide to the 3′-end of a primer in a template-directed extension reaction that then stops. N...

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Hlavní autoři: Hutter, Daniel, Kim, Myong-Jung, Karalkar, Nilesh, Leal, Nicole A., Chen, Fei, Guggenheim, Evan, Visalakshi, Visa, Olejnik, Jerzy, Gordon, Steven, Benner, Steven A.
Médium: Artigo
Jazyk:Inglês
Vydáno: 2010
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On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3858015/
https://ncbi.nlm.nih.gov/pubmed/21128174
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1080/15257770.2010.536191
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