Directional cloning of DNA fragments using deoxyinosine-containing oligonucleotides and endonuclease V

BACKGROUND: DNA fragments carrying internal recognition sites for the restriction endonucleases intended for cloning into a target plasmid pose a challenge for conventional cloning. RESULTS: A method for directional insertion of DNA fragments into plasmid vectors has been developed. The target seque...

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Hlavní autoři: Baumann, Tobias, Arndt, Katja M, Müller, Kristian M
Médium: Artigo
Jazyk:Inglês
Vydáno: BioMed Central 2013
Témata:
Methodology Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3856533/
https://ncbi.nlm.nih.gov/pubmed/24090222
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6750-13-81
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