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Molecular cloning of the human cholecystokinin gene by use of a synthetic probe containing deoxyinosine.
A synthetic DNA based on the known amino acid sequence of the brain/gut peptide cholecystokinin (CCK) was synthesized. This DNA contained deoxyinosines at ambiguous codon positions and was used as a probe to isolate the CCK gene directly from a human genomic library. Nucleotide sequence analysis of...
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| Udgivet i: | Proc Natl Acad Sci U S A |
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| Main Authors: | , , , , , , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
National Academy of Sciences
1985
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC397449/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3856870/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.82.7.1931 |
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