Molecular cloning of the human cholecystokinin gene by use of a synthetic probe containing deoxyinosine.

A synthetic DNA based on the known amino acid sequence of the brain/gut peptide cholecystokinin (CCK) was synthesized. This DNA contained deoxyinosines at ambiguous codon positions and was used as a probe to isolate the CCK gene directly from a human genomic library. Nucleotide sequence analysis of...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Principais autores: Takahashi, Y, Kato, K, Hayashizaki, Y, Wakabayashi, T, Ohtsuka, E, Matsuki, S, Ikehara, M, Matsubara, K
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 1985
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC397449/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3856870/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.82.7.1931
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