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Directional cloning of DNA fragments using deoxyinosine-containing oligonucleotides and endonuclease V
BACKGROUND: DNA fragments carrying internal recognition sites for the restriction endonucleases intended for cloning into a target plasmid pose a challenge for conventional cloning. RESULTS: A method for directional insertion of DNA fragments into plasmid vectors has been developed. The target seque...
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| Hauptverfasser: | , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
BioMed Central
2013
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3856533/ https://ncbi.nlm.nih.gov/pubmed/24090222 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6750-13-81 |
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