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Isolation and Culture of Mouse Primary Pancreatic Acinar Cells
This protocol permits rapid isolation (in less than 1 hr) of murine pancreatic acini, making it possible to maintain them in culture for more than one week. More than 20 x 10(6) acinar cells can be obtained from a single murine pancreas. This protocol offers the possibility to independently process...
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| Main Authors: | , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
MyJove Corporation
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3855917/ https://ncbi.nlm.nih.gov/pubmed/23979477 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/50514 |
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