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Functional Expression of Human NKCC1 from a Synthetic Cassette-Based cDNA: Introduction of Extracellular Epitope Tags and Removal of Cysteines

The Na-K-Cl cotransporter (NKCC) couples the movement of Na(+), K(+), and Cl(−) ions across the plasma membrane of most animal cells and thus plays a central role in cellular homeostasis and human physiology. In order to study the structure, function, and regulation of NKCC1 we have engineered a syn...

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Bibliografiske detaljer
Main Authors: Somasekharan, Suma, Monette, Michelle Y., Forbush, Biff
Format: Artigo
Sprog:Inglês
Udgivet: Public Library of Science 2013
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3855340/
https://ncbi.nlm.nih.gov/pubmed/24339991
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0082060
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