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Functional Expression of Human NKCC1 from a Synthetic Cassette-Based cDNA: Introduction of Extracellular Epitope Tags and Removal of Cysteines
The Na-K-Cl cotransporter (NKCC) couples the movement of Na(+), K(+), and Cl(−) ions across the plasma membrane of most animal cells and thus plays a central role in cellular homeostasis and human physiology. In order to study the structure, function, and regulation of NKCC1 we have engineered a syn...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Public Library of Science
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3855340/ https://ncbi.nlm.nih.gov/pubmed/24339991 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0082060 |
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