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Empirical assessment of sequencing errors for high throughput pyrosequencing data

BACKGROUND: Sequencing-by-synthesis technologies significantly improve over the Sanger method in terms of speed and cost per base. However, they still usually fail to compete in terms of read length and quality. Current high-throughput implementations of the pyrosequencing technique yield reads whos...

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Detalhes bibliográficos
Main Authors: da Fonseca, Paulo GS, Paiva, Jorge AP, Almeida, Luiz GP, Vasconcelos, Ana TR, Freitas, Ana T
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3852801/
https://ncbi.nlm.nih.gov/pubmed/23339526
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1756-0500-6-25
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