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Preparation of Filamentous Actin for Polarized Total Internal Reflection Fluorescence Microscopy (polTIRFM) Motility Assays

Polarized total internal reflection fluorescence microscopy (polTIRFM) can be used to detect the spatial orientation and rotational dynamics of single molecules. polTIRFM determines the three-dimensional angular orientation and the extent of wobble of a fluorescent probe bound to the macromolecule o...

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Bibliografski detalji
Glavni autori: Beausang, John F., Sun, Yujie, Quinlan, Margot E., Forkey, Joseph N., Goldman, Yale E.
Format: Artigo
Jezik:Inglês
Izdano: 2012
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3852664/
https://ncbi.nlm.nih.gov/pubmed/22550306
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/pdb.prot069377
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