The Polarized Total Internal Reflection Fluorescence Microscopy (polTIRFM) Processive Motility Assay for Myosin V

Polarized total internal reflection fluorescence microscopy (polTIRFM) can be used to detect the spatial orientation and rotational dynamics of single molecules. polTIRFM determines the three-dimensional angular orientation and the extent of wobble of a fluorescent probe bound to the macromolecule o...

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Autores principales: Beausang, John F., Sun, Yujie, Quinlan, Margot E., Forkey, Joseph N., Goldman, Yale E.
Formato: Artigo
Lenguaje:Inglês
Publicado: 2012
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Article
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC3852403/
https://ncbi.nlm.nih.gov/pubmed/22661446
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/pdb.prot069393
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