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Rapid Fc glycosylation analysis of Fc fusions with IdeS and liquid chromatography mass spectrometry

We developed a rapid method to analyze Fc glycosylation of Fc fusion proteins, especially those with mutated Fc hinge regions. Fc fusion proteins were digested with IdeS, an IgG specific protease with exosites for substrate recognition and cleavage. The resultant fragments were directly analyzed thr...

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Main Authors: Lynaugh, Heather, Li, Huijuan, Gong, Bing
פורמט: Artigo
שפה:Inglês
יצא לאור: Landes Bioscience 2013
נושאים:
גישה מקוונת:https://ncbi.nlm.nih.gov/pmc/articles/PMC3851216/
https://ncbi.nlm.nih.gov/pubmed/23839239
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4161/mabs.25302
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