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Rapid Fc glycosylation analysis of Fc fusions with IdeS and liquid chromatography mass spectrometry
We developed a rapid method to analyze Fc glycosylation of Fc fusion proteins, especially those with mutated Fc hinge regions. Fc fusion proteins were digested with IdeS, an IgG specific protease with exosites for substrate recognition and cleavage. The resultant fragments were directly analyzed thr...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Landes Bioscience
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3851216/ https://ncbi.nlm.nih.gov/pubmed/23839239 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4161/mabs.25302 |
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