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Approaches to querying bacterial genomes with transposon-insertion sequencing

In this review, we discuss transposon-insertion sequencing, variously known in the literature as TraDIS, Tn-seq, INSeq, and HITS. By monitoring a large library of single transposon-insertion mutants with high-throughput sequencing, these methods can rapidly identify genomic regions that contribute t...

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Detalhes bibliográficos
Main Authors: Barquist, Lars, Boinett, Christine J., Cain, Amy K.
Formato: Artigo
Idioma:Inglês
Publicado em: Landes Bioscience 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3849164/
https://ncbi.nlm.nih.gov/pubmed/23635712
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4161/rna.24765
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