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Subunit Organisation of In Vitro Reconstituted HOPS and CORVET Multisubunit Membrane Tethering Complexes

Biochemical and structural analysis of macromolecular protein assemblies remains challenging due to technical difficulties in recombinant expression, engineering and reconstitution of multisubunit complexes. Here we use a recently developed cell-free protein expression system based on the protozoan...

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Bibliografski detalji
Glavni autori: Guo, Zhong, Johnston, Wayne, Kovtun, Oleksiy, Mureev, Sergey, Bröcker, Cornelia, Ungermann, Christian, Alexandrov, Kirill
Format: Artigo
Jezik:Inglês
Izdano: Public Library of Science 2013
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3846719/
https://ncbi.nlm.nih.gov/pubmed/24312556
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0081534
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