Subunit Organisation of In Vitro Reconstituted HOPS and CORVET Multisubunit Membrane Tethering Complexes

Biochemical and structural analysis of macromolecular protein assemblies remains challenging due to technical difficulties in recombinant expression, engineering and reconstitution of multisubunit complexes. Here we use a recently developed cell-free protein expression system based on the protozoan...

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Enregistré dans:
Détails bibliographiques
Auteurs principaux: Guo, Zhong, Johnston, Wayne, Kovtun, Oleksiy, Mureev, Sergey, Bröcker, Cornelia, Ungermann, Christian, Alexandrov, Kirill
Format: Artigo
Langue:Inglês
Publié: Public Library of Science 2013
Sujets:
Research Article
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC3846719/
https://ncbi.nlm.nih.gov/pubmed/24312556
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0081534
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