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Subunit Organisation of In Vitro Reconstituted HOPS and CORVET Multisubunit Membrane Tethering Complexes

Biochemical and structural analysis of macromolecular protein assemblies remains challenging due to technical difficulties in recombinant expression, engineering and reconstitution of multisubunit complexes. Here we use a recently developed cell-free protein expression system based on the protozoan...

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Bibliografische gegevens
Hoofdauteurs: Guo, Zhong, Johnston, Wayne, Kovtun, Oleksiy, Mureev, Sergey, Bröcker, Cornelia, Ungermann, Christian, Alexandrov, Kirill
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Public Library of Science 2013
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Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3846719/
https://ncbi.nlm.nih.gov/pubmed/24312556
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0081534
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